Chapter 1 Working in a PC2 Laboratory |
Basic Safety Rules |
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Laboratory Maintenance |
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Aseptic Technique and Culture Handling |
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Lighting the bunsen burner video |
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Flaming the loop video |
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Aseptic technique Tubed and Bottled media |
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Autopipettes video |
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Using a spreader to make a lawn plate |
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Anaerobic Jar setup video |
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Chapter 2 Definitions and Glossaries |
General Definitions |
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Bacterial Genetics |
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Morphology |
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Definitions: Prefixes and Suffixes |
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Chapter 3 Microscopy |
Introduction and Basic Technique |
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Special Microscopy Techniques |
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Micrometry |
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Staining Techniques |
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Motility |
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Acid Fast Stain Video |
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Gram Stain Technique |
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Motility Hanging Drop Method |
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India Ink method for capsules video |
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Spore staining technique |
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Chapter 4 Microbial Growth, Death and Enumeration |
Introduction to microbial growth |
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Enumeration |
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Disinfection and Sterilisation |
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Filter sterilisation video |
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MacFarland Opacity Standards video |
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Chapter 5 Biochemical Tests |
Biochemical Tests |
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Bile solubility test video |
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Catalase test video |
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Oxidase test video |
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PYR test video |
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Serum Sugars video |
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X and V Factor test for Haemophilus spp. |
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Chapter 6 Commercial Identification Kits |
Microbact Gram Negative Identification Kit |
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Staphylococcus Latex Agglutination Tests |
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Streptococcus Co-agglutination Tests (Lancefield grouping) |
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Urine Dipslides |
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Urine Dipslides video |
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Lancefield grouping for Streptococci |
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Latex coagulation test for Staphylococci |
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Chapter 7 Antibiotic Sensitivity Testing |
Disc Diffusion including diagnostic discs |
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Minimal Inhibitory and Minimal Bactericidal Concentrations |
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Kirby Bauer disc diffusion test video |
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Chapter 8 Media and Reagents |
Media |
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Stains |
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Reagents |
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Chapter 9 Laboratory Calculations |
Fractions and decimals |
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Exponentials and Powers of 10 |
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Molarity and Concentration |
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pH |
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Dilutions |
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Drawing graphs |
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