Reagents

CATALASE REAGENT

3% H₂O₂, prepared as follows:
H₂O₂ stock solution, 30% w/v 10ml
MQ water 90ml

Store at 4^oC in a light proof bottle.

10% FERRIC CHLORIDE (TDA)

Ferric Chloride (anhydrous) 10gms
Distilled water to 100mls Weight/Volume

(For FeCl₃.6H₂O you require 16.663gms per 100mls. This can be used instead of Ferric Chloride (anhydrous))

KOVACK’S REAGENT

Para Di Methyl amino benzaldehyde 5gms
n - Amyl alcohol (n - pentanol) 75mls
Hydrochloric acid (SpG 1.19) 25mls

1. Heat alcohol and aldehyde in water bath at 50 - 60 ^oC until dissolved
2. Cool and slowly add the HCl
3. Store at 4^oC
   

NITRATE REDUCTASE REAGENTS:

0.1% NAPHTHYL ETHYLENE DIAMINE DiHCl (NITRATE A)

Naphthyl ethylene diamine di HCl 0.1gm
Distilled water 100mls

1. Dissolve naphthyl ethylene diamine diHCl in water
2. Store in dark bottles at 4^oC

0.2% SULPHANILAMIDE (NITRATE B)

Sulphanilamide 0.2 gms
Distilled water 100mls

1. Dissolve sulphanilamide in the water
2. Use the magnetic stirrer - It may take hours!
3. Store at 4^oC
   

OXIDASE STRIPS

N,N,N’,N’, tetramethyl-p-phenylenediamine, made up as a 1% w/v aqueous solution, is used to soak filter paper strips (approximately 1x3 cm) which are then dried and stored in a dark container. The reagent is subject to auto-oxidation and colour change if exposed to light.

VP REAGENTS:

40% POTASSIUM HYDROXIDE (VP1)

KOH 40gms
Creatine 0.3gms
Distilled water to 100mls Weight/Volume

1. Dissolve the KOH in most of the water
2. Dissolve the Creatine in the remaining water
3. Add the two solutions together volumetrically
4. Store at 4^oC

 
ALPHA – NAPHTHOL (VP 2)

Alpha - naphthol 6gms
95% Alcohol to 100mls W/V

1. Dissolve alpha - naphthol in alcohol
2. Store in dark bottles at 4^oC