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Introduction
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Chapter 1 Working in a PC2 Laboratory
Basic Safety Rules
Laboratory Maintenance
Aseptic Technique and Culture Handling
Lighting the bunsen burner video
Flaming the loop video
Aseptic technique Tubed and Bottled media
Autopipettes video
Using a spreader to make a lawn plate
Anaerobic Jar setup video
Chapter 2 Definitions and Glossaries
General Definitions
Bacterial Genetics
Morphology
Definitions: Prefixes and Suffixes
Chapter 3 Microscopy
Introduction and Basic Technique
Special Microscopy Techniques
Micrometry
Staining Techniques
Motility
Acid Fast Stain Video
Gram Stain Technique
Motility Hanging Drop Method
India Ink method for capsules video
Spore staining technique
Chapter 4 Microbial Growth, Death and Enumeration
Introduction to microbial growth
Enumeration
Disinfection and Sterilisation
Filter sterilisation video
MacFarland Opacity Standards video
Chapter 5 Biochemical Tests
Biochemical Tests
Bile solubility test video
Catalase test video
Oxidase test video
PYR test video
Serum Sugars video
X and V Factor test for Haemophilus spp.
Chapter 6 Commercial Identification Kits
Microbact Gram Negative Identification Kit
Staphylococcus Latex Agglutination Tests
Streptococcus Co-agglutination Tests (Lancefield grouping)
Urine Dipslides
Urine Dipslides video
Lancefield grouping for Streptococci
Latex coagulation test for Staphylococci
Chapter 7 Antibiotic Sensitivity Testing
Disc Diffusion including diagnostic discs
Minimal Inhibitory and Minimal Bactericidal Concentrations
Kirby Bauer disc diffusion test video
Chapter 8 Media and Reagents
Media
Stains
Reagents
Chapter 9 Laboratory Calculations
Fractions and decimals
Exponentials and Powers of 10
Molarity and Concentration
pH
Dilutions
Drawing graphs
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